cell tracker blue cmac (Thermo Fisher)

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Thermo Fisher cell tracker blue cmac
Cell Tracker Blue Cmac, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell+tracker+blue+cmac/bio_rxiv__2024__11__01__621145-299-13-17
Average 90 stars, based on 1 article reviews

cell tracker blue cmac - by Bioz Stars, 2026-10

90/100 stars

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other:

Article Title: Ferroptosis contributes to developmental cell death in rice blast.
Article Snippet: Staining procedures and confocal microscopy Conidia inoculated on hydrophobic cover glass for the indicated time period were incubated with 10 μM C11-BODIPY581/591 (Thermo Fisher, D3861) to detect lipid peroxides; 1 μM calcein-AM (Invitrogen, C3099) for iron staining; 2 μg/ml fluorescein diacetate (Sigma, F7378) for viable cell staining; 28 μM Cell TrackerTM Blue CMAC (Invitrogen, C2110) for vacuolar staining; or 10 μM CM-H2DCFDA (Invitrogen, C6827) for cytosolic ROS staining, at room temperature for 20-30 min, followed by two washes with sterile water.

Article Title: Evaluation of paclitaxel-loaded polymeric nanoparticles in 3D tumor model: impact of tumor stroma on penetration and efficacy.
Article Snippet: Trypsin–EDTA 0.5%, fetal bovine serum (FBS), Cell TrackerTM Green CMFDA (5-chloromethyluorescein diacetate), Cell TrackerTM Blue CMAC (7-amino-4-chloromethylcoumarin), and Cell TrackerTM Orange CMTMR (5-(and6)-(((4-chloromethyl)benzoyl)amino) tetramethylrhodamine) were purchased from Life Technologies (Bleiswijk, the Netherlands).

Activity Assay:

Article Title: Peptide Adjuvant to Invigorate Cytolytic Activity of NK Cells in an Obese Mouse Cancer Model
Article Snippet: Then, the Cell Counting Kit-8 (CCK-8; Dojindo Molecular Technologies, Inc., Kumamoto, Japan) was used according to the kit instructions to determine the number of viable cells. .. To analyze anti-cancer cytolytic activity of NK cells, B16F10 cells were pre-labeled with 25 μM Cell Tracker Blue CMAC (Thermo Fisher Scientific) and seeded at 5 × 10 4 cells/well in 48-well plates. ..

Labeling:

Article Title: Role of cytoneme structures and extracellular vesicles in Trichomonas vaginalis parasite-parasite communication
Article Snippet: Briefly, BPH-1 cells were seeded on coverslips in 24-well plates with RPMI(Roswell Park Memorial Institute 1640 medium) culture medium (Invitrogen) and grown to confluence at 37°C and 5% CO 2 . .. Parasites (CDC1132 or G3 strain) were labeled with Cell Tracker Blue CMAC (7-amino-4-chloromethylcoumarin; Invitrogen), added to confluent BPH-1 cells (1:3 parasite: host cell ratio), and exposed to different parasites strains loaded in the inserts in a 1:2 bottom: transwell ratio. .. Plates were then incubated together at 37°C and 5% CO 2 for 60 min. Coverslips were subsequently washed in PBS solution, fixed with 4% paraformaldehyde and mounted on slides with Fluoromont Aqueous Mounting Medium (Sigma).

Article Title: VPS32, a member of the ESCRT complex, modulates adherence to host cells in the parasite Trichomonas vaginalis by affecting biogenesis and cargo sorting of released extracellular vesicles.
Article Snippet: Trichomonas vaginalis is a common sexually transmitted extracellular parasite that adheres to epithelial cells in the human urogenital tract.. Extracellular vesicles (EVs) have been described as important players in the pathogenesis of this parasite as they deliver proteins and RNA into host cells and modulate parasite adherence.. EVs are heterogeneous membrane vesicles released from virtually all cell types that collectively represent a new dimension of intercellular communication.

Isolation:

Article Title: Collagen binding adhesin restricts Staphylococcus aureus skin infection
Article Snippet: .. Briefly, neutrophils were isolated as described above (4X10 6 /mL) and stained with Cell Tracker Blue CMAC (Thermo Fisher, Cat No: C2110). .. Opsonized bacteria labelled with Syto-9 (Invitrogen, Cat No: S34854) were washed and incubated with neutrophils (1:25) for 20 minutes, similarly to method described above.

Staining:

Saline:

Article Title: An injectable, self-healing, 3D printable, double network co-enzymatically crosslinked hydrogel using marine poly- and oligo-saccharides for wound healing application
Article Snippet: In this study, we designed dual network hydrogels with antioxidant and antibacterial activities using marine polyand oligosaccharides with skin wound healing potential.. The synergy between dual enzymatic cocrosslinking based on glucose oxidize (GOx)/horseradish peroxidase (HRP) and electrostatic interaction between positively charged chitooligosaccharides (COS) and phenolated chitosan with negatively charged phenolated alginate formed a hydrogel.. The Gel-COS hydrogels exhibited toughness, self-healing, moldability, injectability, and 3D printability.

Expressing:

Article Title: Agrin-Matrix Metalloproteinase-12 axis confers a mechanically competent microenvironment in skin wound healing
Article Snippet: .. One hundred thousand Human Dermal Microvascular endothelial cells (HDMEC) cells were pre-labeled overnight with Cell Tracker Blue CMAC (7-amino-4-chloromethyl coumarin-Invitrogen) and mixed with equal amounts of GFP expressing BJ cells. ..

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